I'm going to assume the original Ensolite (closed cell foam with textured vinyl surface) is still in place on the walls. It's as good as any alternative that is practical to install on the curved surfaces of an egg trailer. Not much you can do there. There's no magic about insulation. The R-value is a function of thickness, and there's not room to add much of that in a tiny trailer. You would end up with a bed that's too short, a ceiling that's too low...
Trillium is known for its large banks of
jalousie windows. I agree that insulating them will give the most improvement for the least effort and cost. I'd go with Reflectix (foil bubble wrap) for its radiant properties. Pieces of rigid foam in any roof vents will help. Interlocking foam tiles on the floor. Look for any other air gaps you can seal.
You'll want to leave a little outside air flow in cold weather, though, or you'll have condensation issues.
What kind of furnace/heater are you using? It doesn't take much to heat a small trailer.